REAL-TIME PCR

The DNA Core runs an ABI 7900HT Real-Time PCR System

This System can accommodate any real-time PCR application. This new high-throughput system contains robotics enabling multiple 384 or 96 well plates to be loaded automatically. Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction. The software is designed to perform all necessary calculations including dissociation curve analysis, absolute quantitation, and relative quantitation using standard curve or comparative CT algorithms.

POSSIBLE APPLICATIONS INCLUDE:

• Allelic Discrimination:SNP Analysis using fluorescent 5' nuclease assay
• Measurement of gene expression levels (gene expression quantitation)
• Quantitative PCR
• Array Verification
• Gene Expression
• Analysis of mRNA Expression
• Mutation Detection

INFORMATION:

Essentials of Real Time PCR - by Applied Biosystems

Real-Time PCR Vs. Traditional PCR - by Applied Biosystems

Current challenges of real-time PCR quantitation - by Jorge A. Garces, Ph.D.

SNPs – Powerful Toolsfor Association Studies - by Applied Biosystems

REQUISITION FORM: (WORD) (PDF)

In order to facilitate easier scheduling for all users, please call the DNA Core at 732-235-4934 to make an appointment to drop off your PCR samples. Plates must be dropped off by 1:00 pm to be run that day. Plates dropped off later in the day may be held over for the next business day.

CHARGES:

$10.00 per plate for CINJ members

$25.00 per plate UMDNJ or Rutgers

Please stop by or call the Core for details and assistance in setting up your experiments. We can help you with your assay design, and assist you with using ABI's File Builder or Primer Express softwares.