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Laboratory Safety Incidents Sorted by Key Instruction Concept

bullet.gif (565 bytes)   Add chemicals in correct order to minimize splattering
bullet.gif (565 bytes)   Be aware of the possibility of pressurization when working
     with cryogenic liquids

bullet.gif (565 bytes)   Certain chemicals must be allowed to vent or they could
     explode.

bullet.gif (565 bytes)   Chemical containers should be triple rinsed and dry
     before being used for waste accumulation

bullet.gif (565 bytes)   Consider periodic testing of animal colonies, regardless
    of source
bullet.gif (565 bytes)   Consider shielding for operations involving vacuum or
     pressurization
bullet.gif (565 bytes)   Control sources of contamination
bullet.gif (565 bytes)   Cryogenic Liquids expand greatly in air and may cause
      asphyxiation if used with inadequate ventilation.
bullet.gif (565 bytes)   Do not defeat machine safety interlocks
bullet.gif (565 bytes)   Don't leave reaction unattended
bullet.gif (565 bytes)   Do not refill gas cylinders with highly reactive or corrosive
     compounds, including the original compound.

bullet.gif (565 bytes)   Don't rush
bullet.gif (565 bytes)   Do not transfer liquified gases from one tank to another
     the first time without supervision
bullet.gif (565 bytes)   Do not work around energized, exposed conductors
bullet.gif (565 bytes)   Ensure adequate ventilation
bullet.gif (565 bytes)   Flammable liquids must only be stored in refrigerators
     and freezers which have no internal ignition sources.
bullet.gif (565 bytes)   Limit the amount of highly reactive, toxic, or flammable
     chemicals to the quantity necessary for planned
     experiments

bullet.gif (565 bytes)   Lower the hood sash
bullet.gif (565 bytes)   Many biological safety cabinets recirculate air.  The motor
     is an ignition source.  Use of flammable gases or vapors
     may cause a fire
bullet.gif (565 bytes)   Practice good personal hygiene for prevention of disease
bullet.gif (565 bytes)   Read the label
bullet.gif (565 bytes)   Recognize location of nearest ignition sources when using
     flammable liquids
bullet.gif (565 bytes)   Replace worn components
bullet.gif (565 bytes)   Review hazards of chemicals before starting
bullet.gif (565 bytes)   Review Risk Assessment when scaling up reactions
bullet.gif (565 bytes)   Secure hoses/tubing
bullet.gif (565 bytes)   Separate animal use areas from "people areas"
bullet.gif (565 bytes)   Special precautions and training are needed for work
     involving sheep
bullet.gif (565 bytes)   Treat research animals as if they may have zoonotic
     disease
bullet.gif (565 bytes)   Use appropriate personal protective equipment
bullet.gif (565 bytes)   Use engineering controls
bullet.gif (565 bytes)   Use sealed rotors or safety cups when centrifuging blood,
     pathogens or other potentially infectious material.

bullet.gif (565 bytes)   Use oxygen monitors when there is a potential for liquid
     nitrogen build-up

bullet.gif (565 bytes)   Vacate room and wait at least 30 minutes for infectious
     aerosols to settle before attempting a clean-up
bullet.gif (565 bytes)   Vent chemicals that off-gas
bullet.gif (565 bytes)   Wear long pants and shoes in the lab
bullet.gif (565 bytes)   Wear safety glasses while in the laboratory -even when
     performing non laboratory work

bullet.gif (565 bytes)   Work at least 6" inside chemical hood
bullet.gif (565 bytes)   Written emergency procedures should be readily available
     for potential accidents/incidents that can occur in the lab.

bullet.gif (565 bytes)   Work restrictions may be appropriate for
     immunocompromised persons

 

Add chemicals in the correct order to minimize splattering  (top)

Mixing Diaminopropane and Potassium Hydroxide - Explosion

Be aware of the possibility of pressurization when working with cryogenic liquids  (top)

Glass Flask Ruptures - Possibly Overpressurization by Liquid Nitrogen

Reseracher Blinded in One Eye From Cryotube Explosion

Investigator exposed to infectious material in Cryotube Explosion

Certain chemicals must be allowed to vent or they could explode.  (top)

Laboratory Glass Cleaning Reagent Incident

Alert: Formic Acid Explosion and Explosive Laboratory Chemicals  The Australian University, Human Resources Occupational Health and Safety, December 1997

Two Explosions Involving Aqua Regia

Chemical containers should be triple rinsed and dry before being used for waste accumulation  (top)

Glass Waste Bottle Ruptures, Possible Reaction of Incompatible Chemical Wastes

Consider periodic testing of animal colonies, regardless of source  (top)

Viral Meningitis in a Researcher  (top)

Consider shielding for operations involving vacuum or pressurization  (top)

Glass Flask Ruptures - Possibly Overpressurization by Liquid Nitrogen

Reseracher Blinded in One Eye From Cryotube Explosion

Control Sources of Contamination  (top)

Stirred Reaction Flask Explosion

Cryogenic Liquids expand greatly in air and may cause asphyxiation if used with inadequate ventilation.  (top)

Liquid NitrogenAsphyxiation - MRI

Lessons From the Fatality at AAHL, Commonwealth Scientific and Industrial Research Organization

Do Not Defeat Machine Safety Interlocks  (top)

Electrical Shock from Laser Power Supply

Don't Leave Reaction Unattended  (top)

Stirred Reaction Flask Explosion

Don't Rush  (top)

Autoclave Scalding

Do not transfer liquified gases from one container to another the first time without supervision  (top)

Liquid Nitrogen Splash

Do not work around energized, exposed conductors  (top)

Electrical Shock from Electrophoresis Unit

Electrical Shock from Laser Power Supply

Do not refill gas cylinders with highly reactive or corrosive compounds.  (top)

Ruptured Gas Cylinder Destroys Laboratory Hood 

Ensure Adequate Ventilation  (top)

Liquid Nitrogen Asphyxiation

Lessons From the Fatality at AAHL, Commonwealth Scientific and Industrial Research Organization

Flammable Liquids must only be stored in refrigerators and freezers which have no internal ignition sources.  (top)

Lab Freezer Explodes

Limit the amount of highly reactive, toxic, or flammable chemicals to the quantity necessary for planned experiments  (top)

Ruptured Gas Cylinder Destroys Laboratory Hood 

Lower the Hood Sash  (top)

Stirred Reaction Flask Explosion

Chemical Solution Preparation Explosion

Chemical Exposure To The Eye In Spite of Goggles

Phenyl Azide Solution Erupts During Vacuum Distillation

Mixing Diaminopropane and Potassium Hydroxide - Explosion

Many biological safety cabinets recirculate air.  The motor is an ignition source.   Flammable vapors and gases may build up and cause a fire.

Two Flaming Loop Fires, both inside a  BSC

Flame Sterilizing Incident

Unattended Use of a Bunsen Burner inside a BSC 

Practice Good Personal Hygiene for prevention of Disease  (top)

Cryptosporidium in Veterinary Students (top)

Read the Label  (top)

Arsine Gas Release

Recognize location of nearest ignition sources when using flammable liquids  (top)

Carbon Disulfide Fire 

Replace Worn Components  (top)

Hotplate Fire:   A Report on a Fire Incident During Sublimation of a Colorant Intermediate using a Cold Finger and Oil Bath

Review Hazards of Chemicals Before Starting (top)

Chemical Solution Preparation Explosion

Review Risk Assessment when Scaling Up Reactions  (top)

Explosive Decomposition of an Organic Azide

Glass flask Rupture During Ozonolysis

Mixing Diaminopropane and Potassium Hydroxide - Explosion

Secure hoses/tubing   (top)

Hotplate Fire:   A Report on a Fire Incident During Sublimation of a Colorant Intermediate using a Cold Finger and Oil Bath

Separate animal use areas from "people areas"  (top)

Q Fever in Medical School Personnel (top)

Special precautions and training are needed for work involving sheep  (top)

Q Fever in Medical School Personnel  (top)

Treat all research animals as if they may have zoonotic disease  (top)

Cryptosporidium in Veterinary Students  (top)

Viral Meningitis in a Researcher  (top)

Use Appropriate Personal Protective Equipment  (top)

Fire From Diethyl Ether Spill

Wrong Gloves Lead To Chemical Burn

Chemical Solution Preparation Explosion

Glass Waste Bottle Ruptures, Possible Reaction of Incompatible Chemical Wastes

Reseracher Blinded in One Eye From Cryotube Explosion

Liquid Nitrogen Splash

Mixing Diaminopropane and Potassium Hydroxide - Explosion

Use Engineering Controls    (top)

Arsine Gas Release

Hotplate Fire:   A Report on a Fire Incident During Sublimation of a Colorant Intermediate using a Cold Finger and Oil Bath


Explosive Decomposition of an Organic Azide

Semiconductor Experiment Explosion:  Failure to Manually Purge Hazardous Gases

Use oxygen monitors when there is a potential for liquid nitrogen build-up (top)

Lessons From the Fatality at AAHL, Commonwealth Scientific and Industrial Research Organization

Vent chemicals that off-gas   (top)

Formic Acid Explosion and Explosive Laboratory Chemicals

Two Explosions Involving Aqua Regia

Laboratory Glass Cleaning Reagent Incident

Wear long pants and shoes in the lab (top)

Fire From Diethyl Ether Spill

Wear safety glasses while in the laboratory - even when performing non-laboratory work   
   (top)

Glass Waste Bottle Ruptures, Possible Reaction of Incompatible Chemical Wastes

Work at Least 6" Inside The Chemical Hood  (top)

Fire From Diethyl Ether Spill

Work Restrictions May be Appropriate for Immunocompromised Persons  (top)

Cryptosporidium in Veterinary Students (top)

Q Fever in Medical School Personnel (top)

Septicemia Following a Dog Bite (top)

 


Revision Date:  8/18/2003
url:  http://www2.umdnj.edu/eohssweb/aiha/accidents/lesson.htm

 

 

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