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Donald A. Winkelmann, Ph.D.
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Position:
Associate
Professor of Pathology and Laboratory Medicine, Robert Wood Johnson
Medical School,
Administrative:
Chief,
Division of Medical Informatics,
UMDNJ - Robert Wood Johnson Medical
School
Co-Director of
graduate programs in Molecular Biosciences, Rutgers/UMDNJ
Department: Pathology and Laboratory Medicine
Lab Url:
http://pleiad.umdnj.edu/~daw/index.htm
Phone Number: (732) 235-4759
Fax Number: (732) 235-4825
Email
Address: winkelma@umdnj.edu |
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Clinical and Research
Interests |
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The focus of our research
is the study of macromolecular structure and assembly with our efforts
concentrated on the analysis of the protein myosin and its interaction
with actin. Actin and myosin are highly conserved proteins that
participate in determination of cell shape, cellular motility,
cytokinesis and contractility. Our research uses the techniques of
protein biochemistry, molecular genetics, immunochemistry, electron
microscopy and crystallography to analyze myosin structure, assembly and
function. These studies have contributed to the current understanding of
myosin structure and have culminated in the determination of the
structure of the myosin motor domain at 2.8 Å resolution. The molecular
model of myosin is revealing exciting new insights into the fundamental
mechanism of biological energy transformation. These structural studies
are complemented by the use of molecular genetics and site directed
mutagenesis of cloned myosin genes. In addition to its role in
generating force and motion in all eukaryotic cells, myosin is a major
structural component of the contractile organelle of muscle and
non-muscle cells. We are investigating this role by expression of GFP-myosin
chimeras and fluorescent microscopy of living cardiac and skeletal
muscle cells. |
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Training |
 | University
of Wisconsin-Milwaukee, WI, B.S., Chemistry, 1973 |
| University
of Wisconsin, Madison, WI, Ph.D., Biochemistry, 1980 |
| Structural
Biology Laboratory, Rosenstiel Center, Brandeis University,
Waltham, MA 1980-86
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Honors and Awards |
1973 B.S. (Magna Cum Laude) Honors in Chemistry
1977 - 1979 NIH National Research Service Award trainee
1980- 1982 Paul Cohen Postdoctoral Fellow, the Muscular
Dystrophy
1983 - 1985 Research Fellowship, The Medical Foundation, Inc.,
Boston
1987 - 1992 Established
Investigator, The American Heart
Association |
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Certifications |
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Licensure |
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N.A. |
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N.A. |
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Office Address |
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Mailing Address |
Department of
Pathology & Laboratory Medicine
675 Hoes Lane
UMDNJ - Robert Wood Johnson Medical School
Piscataway, NJ 08854, USA |
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Selected
Publications |
- Xiang, X., G. Han, D.A. Winkelmann,
W. Zuo and N.R. Morris. (2000) dynamics of cytoplasmic dynein in
living cells and the effect of a mutation in the dynactin complex
actin-related protein Arp1.
Current Biology 10: 603-606.
- Srikakulam, R. and D.A. Winkelmann.
(1999) Myosin II Folding is Mediated by a Molecular chaperonin.
J. Biol.
Chem. 274:27265-27273.
- Colognato, H., D.A. Winkelmann and
P.D. Yurchenco. (1999) Laminin Polymerization Induces a
receptor-cytoskeleton network.
J. Cell
Biol. 145:619-631.
- Riveline, D., A. Ott, F. Julicher,
D.A. Winkelmann, O. Cardoso, J-J. Lacapere, S. Magnusfottir, J-L.
Viovy, L. Gorre-Talini and J. Prost. (1998) Acting on actin: The
electric motility assay. Eur. J. Biophys. 27:403-408.
- Birk, D.E., Zycband, E.I., Woodruff,
S., Winkelmann D.A. and Trelstad, R.L. (1997). Collagen
fibrillogenesis in situ: Fibril segments become long fibrils as the
developing tendon matures. Dev. Dynamics. 208:291-298.
- Kinose, F., Wang, S.X., Kidambi, U.,
Moncman , C.L. and Winkelmann, D.A. (1996). Glycine 699 is pivotal for
the motor activity of skeletal muscle myosin.
J. Cell
Biol. 134:895-909.
- Winkelmann, D.A., Bourdieu, L., Ott,
A., Kinose, F. and Libchaber, A. (1995). The flexibility of myosin
attachment to surfaces influences F-actin motion.
Biophys. J. 68: 2444-2453.
- Bourdieu, L. Duke, T., Elowits, M.B.,
Winkelmann, D.A., Leibler, S. and Libchaber, A. (1995). Spiral defects
in motility assays: a measure of motor protein force. Phys. Rev. Lett.
75:176-179.
- Bourdieu, L., Magnasco, M.O.,
Winkelmann, D.A., Libchaber, A. (1995). Actin filaments on myosin
beds, the velocity distribution. Phys. Rev. E, 52:6573-6579.
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Postdoctoral Position Available |
None
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Research Positions Available |
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None
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