UMDNJ - ROBERT WOOD JOHNSON MEDICAL SCHOOL
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Storage diseases
Cilia disorders (immotile cilia diseases)
| Infectious diseases: viral diseases |
Whipple's disease and microsporidia
Miscellaneous: congenital bullous skin diseases
Tissue samples for electron microscopy should be submitted as 1 mm cubes in 2% buffered glutaraldehyde. Buffered formalin may be adequate; mercuric fixatives (B5, Zenker's) cannot be used. In selected cases, tissue can be retrieved from paraffin blocks and processed for EM.
In addition to tissue samples, representative H & E-stained slides as well as a completed EM requisition should be sent. Please also provide the results of any other studies (i.e. immunohistochemistry) to aid in our diagnostic evaluation. Tissue processing for EM requires 2-3 days. Results are reported by telephone, fax, and a final printed report.
Samples can be shipped at
ambient temperature. We can provide glutaraldehyde fixative, EM requisitions,
and pre-printed airbills upon request. Samples are accepted Monday through
Friday.
Samples in 2% glutaraldehyde may be multiple, but should not exceed 1mm in
dimension because of the penetrating abilities of this fixative. If
glutaraldehyde is unavailable please contact us by phone 615-322-3070 or by
e-mail, and we will provide you with specimen containers containing this
fixative. Formalin, or other fixed tissue is also usually adequate if no
glutaraldehyde is available. Sometimes a paraffin embedded specimen can be used,
but with some sacrifice of quality.
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