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Research Interest:
Glutamate receptor up and down regulation in brain
Description:
Suitable agonist or antagonist for a subtype of glutamate receptor
is given to rats either intraperitonially or directly infused on
to the brain surface by osmotic pumps for seven days. The number
of receptors is estimated from the specific binding of radiolabelled
ligands to the up or down regulated brain sections in vitro. Regional
cerebral O2 consumption (cryo-micro-spectrophotometry) is determined
for similarly treated rats. The effect of glutamate receptor subtype
up or down regulation on regional cerebral O2 consumption is then
determined under resting and challenged (glutamate receptor stimulated)
conditions. Drug development for blocking NMDA or AMPA glutamate
receports must consider the possibility of receptor upregulation.
Publications:
Weiss HR, AK Sinha and X Lu. Effect of up-regulation of NMDA receptors
on cerebral O2 consumption and blood flow in rat. Brain Res. 730:193-198,
1996.
Lu X, AK Sinha, HR Weiss. Effects of excitatory amino acids on
cerebral oxygen consumption and blood flow in rat. Neurochem. Res.
22:705-711, 1997.
Sinha AK, AH Mirza, X Liu, OZ Chi, and HR Weiss. Effect of upregulation
of AMPA glutamate receptors on cerebral O2 consumption and blood
flow in rat. Brain Res. 842:230-232, 1999.
Staff:
Harvey Weiss, Ph.D., Professor, Collaborator
Xia Liu, Adjunct instructor
Graduate Program Membership:
Physiology and Neurobiology
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