Alexey G. Ryazanov, Ph.D.
Professor Department of Pharmacology
Robert Wood Johnson Medical School 
Office: RWJMS/Piscataway R443 
Telephone: (732) 235-5526 
FAX: (732) 235-4073 
E-mail: ryazanag@umdnj.edu
Research Interests Recent Publications Lab Staff

 

Alpha-kinases, channel-kinases and cell signaling

Research Description:
We have recently discovered a new class of protein kinases -- alpha-kinases. Alpha-kinases play an important role in the control of protein synthesis, cell growth and differentiation. Two alpha-kinases that we recently identified represent a novel type of signaling molecule that consists of a protein kinase fused to an ion-channel. Why would a kinase and ion-channel be contained in one molecule ? Is the channel activity controlled by the kinase, or is the kinase controlled by ion flow through the channel ? We are using molecular biological, biochemical, and bioinformatic approaches as well as transgenic animals to investigate the structure and function of these fascinating molecules. 

An online database of all known alpha-kinases and related links is available. (New Version) (Old Version)

 

  • Dorovkov, M.V. and Ryazanov, A.G.(2004) Phosphorylation of Annexin I by TRPM7 Channel-Kinase. J. Biol. Chem. 279: 50643-50646. . PubMed - PDF

  • Drennan, Diana and Ryazanov, Alexey G. Alpha-kinases: analysis of the family and comparison with conventional protein kinases, Progress in Biophysics and Molecular Biology, Volume 85, Issue 1, May 2004, Pages 1-32. PubMed - PDF

  • Ryazanova, Lillia V., Dorokov, Maxim V., Ansari, Athar, and Ryazanov, Alexey G.
    Characterization of the Protein Kinase Activity of TRPM7/ChaK1, a Protein Kinase Fused to the Transient Receptor Potential Ion Channel J. Biol. Chem. 279: 3708-3716. JBC - PDF

  • Dorovkov M.V., Pavur K.S., Petrov A.N., Ryazanov A.G. (2002) Regulation of elongation factor-2 kinase by pH. Biochemistry 41(45):13444-50. PubMed - PDF

  • Ryazanov, A.G. (2002) Elongation factor-2 kinase and its newly discovered relatives. FEBS letters 514: 26-29.  PubMed - PDF

  • Ryazanov, A.G., Nefsky, B.S. (2002) Protein turnover plays a key role in aging. Mech. Ageing Dev. 123, 207-213. PubMed - PDF

  • Ryazanova, L.V., Pavur, K.S., Petrov, A.N., Dorovkov, M.V. and Ryazanov, A.G. (2001) Novel type of signaling molecules: protein kinases covalently linked with ion channels. Mol. Biol. 35: 271-283.  PubMed - PDF

  • Alberdi F., Dadone J., Ryazanov A.G., Isenberg D.A., Ravirajan C., Reichlin M. (2001) Cross-reaction of lupus anti-dsDNA antibodies with protein translation factor EF-2. Clin. Immunol. 98: 293-300.  PubMed - PDF

  • Pavur, K.S., Petrov, A.N., and Ryazanov, A.G. (2000) Mapping the functional domains of elongation factor-2 kinase. Biochemistry 39:12216-12224.  PubMed - PDF

  • Ryazanov, A.G., Pavur, K.S. and Dorovkov, M.V.(1999) Alpha-kinases: a new class of protein kinases with a novel catalytic domain. Current Biology 9: R43-R45.  PubMed

  • Enkemann, S.A., Pavur, K.S., Ryazanov, A.G., and Berger, S.L. (1999) Does prothymosin a affect the phosphorylation of elongation factor-2? J. Biol. Chem. 274: 18644-18650.  PubMed - PDF

  • Ryazanov, A.G., Ward, M.D., Mendola, C.E., Pavur, K.S., Dorovkov, M.V., Wiedmann, M., Erdjument-Bromage, H., Tempst, P., Parmer, T.G., Prostko, C.R., Germino, F.J. and Hait, W.N. (1997) Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. Proc. Natl. Acad. Sci. USA 94:4884-4889. PubMed - PDF